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Handling ITS reads with variable lengths: Should ASVs be normalized to avoid redundancy?

Status: Open.

#2166 In benjjneb/dada2;

· CarolTMartins opened on Dec 5, 2025

removeBimeraDenovo freezing then crashing

Status: Open.

#2165 In benjjneb/dada2;

· damianasrojas opened on Nov 20, 2025

[HELP] All ASVs occurring only once across samples - 16S amplicon

Status: Open.

#2164 In benjjneb/dada2;

· Ka1wenSun opened on Nov 13, 2025

Is merging after denoising really the way to go?

Status: Open.

#2163 In benjjneb/dada2;

· hjarnek opened on Nov 13, 2025

Questions on filter and trim removal of reads

Status: Open.

#2162 In benjjneb/dada2;

· cdesouza02 opened on Nov 12, 2025

[Help] DADA2 error rates could not be estimated for iSeq data — how to fix in R?

Status: Open.

#2160 In benjjneb/dada2;

· ShuoyunJ opened on Oct 29, 2025

Clarify comment about 'On Windows set multithread=FALSE' in tutorial

Status: Open.

#2158 In benjjneb/dada2;

· Yixuan39 opened on Oct 28, 2025

Should you use pooled chimera filtering on a merged dataset?

Status: Open.

#2157 In benjjneb/dada2;

· hjarnek opened on Oct 27, 2025

Different outputs (track read file) after running dada2 script twice on same samples on HPC

Status: Open.

#2156 In benjjneb/dada2;

· rgn06 opened on Oct 9, 2025

Majority of low prevalence ASVs on high quality sequencing - impossible dataset

Status: Open.

#2155 In benjjneb/dada2;

· dgiovannelli opened on Oct 7, 2025

Rarefy the ASVs before assigning taxonomy?

Status: Open.

#2154 In benjjneb/dada2;

· catguerra opened on Oct 6, 2025

High error frequency with high quality scores

Status: Open.

#2151 In benjjneb/dada2;

· kathschmidt opened on Sep 19, 2025