bbtools tool cannot be found. Please check the 'path' and 'command' parameters provided in the config file or make sure the tool is available in your working environment.

[Jiangjiangzhang6]

when I was run the busco, its show bbtools tool was not found
2023-04-10 14:48:04 INFO: ***** Start a BUSCO v5.4.6 analysis, current time: 04/10/2023 14:48:04 *****
2023-04-10 14:48:04 INFO: Configuring BUSCO with local environment
2023-04-10 14:48:04 WARNING: Running Auto Lineage Selector as no lineage dataset was specified. This will take a little longer than normal. If you know what lineage dataset you want to use, please specify this in the config file or using the -l (--lineage-dataset) flag in the command line.
2023-04-10 14:48:04 ERROR: The parameter "in (--in)" was not provided. Please add it in the config file or provide it through the command line
2023-04-10 14:48:04 ERROR: BUSCO analysis failed!
2023-04-10 14:48:04 ERROR: Check the logs, read the user guide (https://busco.ezlab.org/busco_userguide.html), and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues

So I search this issue in google but not found the solution way , how could I do to deal with this problem

hoping for your replay

thank you

[Jiangjiangzhang6]

sorry , this was the error emssage
2023-04-10 14:44:25 INFO: ***** Start a BUSCO v5.4.6 analysis, current time: 04/10/2023 14:44:25 *****
2023-04-10 14:44:25 INFO: Configuring BUSCO with local environment
2023-04-10 14:44:25 INFO: Mode is genome
2023-04-10 14:44:25 INFO: Downloading information on latest versions of BUSCO data...
2023-04-10 14:44:38 INFO: Input file is /public/home/zhangjiangjiang/jjzhang/yan/genome/hemp/bama/S3/S3/SZ-3/02.hic/02.phased/01.hap1/modif/third/gap_filling/chr11/update.chr11.fasta
2023-04-10 14:44:38 INFO: Using local lineages directory /public/home/zhangjiangjiang/software/busco-master/embryophyta_odb10
2023-04-10 14:44:40 ERROR: bbtools tool cannot be found. Please check the 'path' and 'command' parameters provided in the config file or make sure the tool is available in your working environment.
2023-04-10 14:44:40 ERROR: BUSCO analysis failed!
2023-04-10 14:44:40 ERROR: Check the logs, read the user guide (https://busco.ezlab.org/busco_userguide.html), and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues

[handesome]

sorry , this was the error emssage 2023-04-10 14:44:25 INFO: ***** Start a BUSCO v5.4.6 analysis, current time: 04/10/2023 14:44:25 ***** 2023-04-10 14:44:25 INFO: Configuring BUSCO with local environment 2023-04-10 14:44:25 INFO: Mode is genome 2023-04-10 14:44:25 INFO: Downloading information on latest versions of BUSCO data... 2023-04-10 14:44:38 INFO: Input file is /public/home/zhangjiangjiang/jjzhang/yan/genome/hemp/bama/S3/S3/SZ-3/02.hic/02.phased/01.hap1/modif/third/gap_filling/chr11/update.chr11.fasta 2023-04-10 14:44:38 INFO: Using local lineages directory /public/home/zhangjiangjiang/software/busco-master/embryophyta_odb10 2023-04-10 14:44:40 ERROR: bbtools tool cannot be found. Please check the 'path' and 'command' parameters provided in the config file or make sure the tool is available in your working environment. 2023-04-10 14:44:40 ERROR: BUSCO analysis failed! 2023-04-10 14:44:40 ERROR: Check the logs, read the user guide (https://busco.ezlab.org/busco_userguide.html), and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues

same error，did you solved the error？Thanks