package main
import (
//<<Imports>>
)
func main(){
//<<Main function>>
}
//<<Declare options>>
//<<Set usage>>
//<<Parse options>>
//<<Run \texttt{Intersect}>>
//<<Print the results>>
optR := flag.String("r", "", "reference sequence")
optP := flag.Float64("p", 0.975,
"threshold p-value for a shustring length")
optN := flag.Bool("n", false, "print segregating sites (Ns) " +
"in the output sequences")
optF := flag.Float64("f", 1.0,
"intersection sensitivity threshold")
optT := flag.Int("t", 1, "number of threads")
u := "intersect [option]..."
p := "Find common homologous regions in a set of genomes"
e := "intersect -r subject.fna query1.fna query2.fna ..."
clio.Usage(u, p, e)
"github.com/evolbioinf/clio"
flag.Parse()
//<<Check the reference>>
//<<Read reference's contigs>>
//<<Set parameters>>
if *optR == "" {
fmt.Fprintf(os.Stderr,
"please specify the reference sequence\n")
os.Exit(1)
}
f, _ := os.Open(*optR)
referenceContigs := fastautils.ReadAll(f)
f.Close()
"github.com/ivantsers/fastautils"
//<<Interpret *optP>>
//<<Interpret *optF>>
//<<Create a Parameters struct>>
if *optP > 1 || *optP < 0 {
fmt.Fprintf(os.Stderr,
"can't use %v as a sensitivity threshold\n",
*optF)
os.Exit(1)
}
if *optF > 1.0 || *optF <= 0.0 {
fmt.Fprintf(os.Stderr,
"can't use %v as a sensitivity threshold, " +
"please use a value in the interval (0,1]",
*optF)
os.Exit(1)
}
parameters := chr.Parameters{
Reference: referenceContigs,
QueryPaths: flag.Args(),
Threshold: *optF,
ShustrPval: *optP,
PrintN: *optN,
NumThreads: *optT,
}
"github.com/ivantsers/chr"
isc := chr.Intersect(parameters)
for _, seq := range(isc) {
fmt.Fprintf(os.Stdout, "%s\n", seq)
}